2 \V. J. BAUMGARTNER. 



limate plus ten per cent, acetic acid proved fairly good but mate- 

 rial thus fixed shrank too much during imbedding. 



The best stains were Heidenhain's iron-haematoxylin and 

 Flemming's triple stain. 



Besides the material used before, I have collected the common 

 black field cricket about Chicago, and Woods Roll, Mass. The 

 specimens from Lawrence, Kans., were called Gryllus assimilis 

 after comparing them with specimens in the University collec- 

 tion there. Mr. F. E. Lutz called the same specimens Gryllus 

 luctnosns after comparing them with forms about Chicago. I shall 

 not enter into a discussion as to what are good species among 

 Gryllus or what species are found in the different localities, but 

 shall continue to use the name Gryllus assimilis for the species of 

 the common black field cricket until the species are better differ- 

 entiated. 



The testes of the field cricket were described in the former 

 paper and that of Gryllus domcsticus corresponds very closely with 

 it as to shape, size, location and arrangement of follicles and cysts. 



The number of chromosomes in the field cricket is twenty- 

 nine in the spermatogonia and not twenty-three as suggested in 

 the first paper. Then I determined the number by counting the 

 chromosomes in the equatorial plate of the first spermatocyte 

 division and there the counting is difficult for the reason that the 

 chromosomes usually do not enter the equatorial plate at the 

 same time. I have made a great many counts since both in 

 spermatogonia and spermatocytes and feel confident that the 

 numbers are twenty-nine, and fourteen or fifteen respectively. 

 In G. domestic-its there are but twenty -one in the spermatogonia 

 and ten or eleven in the spermatocytes. Because of this smaller 

 number G. domcsticus is more favorable for making drawings. 



The stages with their relation and limits and the use of terms 

 is indicated in the former paper. One point stated there I wish 

 to emphasize as it is even more marked in G. domesticus than in 

 G. assimilis, namely the fact that the cells in one cyst are not all 

 in exactly the same stage of development. Some of the cells, 

 usually on one side of the cyst, are a little in advance and others 

 lag a little. This makes it possible to see the exact sequence of 

 stages by comparing the cells of the cysts in different follicles. 



