244 



HAROLD D. CLAYBERG. 



Cu. mm. 

 liter 



630 

 600 

 570 

 540 

 510 

 480 

 450 

 420 

 390 



360 

 330 

 300 

 270 

 240 

 210 

 ISO 

 150 

 120 

 90 

 60 



CHART III. 



Death Curve of Lepomis Humilis in Chloroform. 

 (CHCla.) 

















* * 



\" ' ' ''' I ... 







10 20 30 40 50 60 70 80 90 100 



Time Until Death Minutes. 



CHART III. (Chloroform death curve.) Each point represents a separate 

 experimental result. The poison is obviously very toxic as a concentration of 

 less than .09 c.c. per liter kills in an hour. There is a sudden change in the curve 

 between the concentrations of 100 and 200 cu. mm. per liter as if a new factor 

 suddenly entered into the determination of the curve below the limits indicated. 

 Only four of the fifteen fish used were above 6.2 grams and these were all used to 

 determine the curve above 190 c. mm. /liter. Hence no classes by weight could be 

 separated. 



