74 N. H. COWDRY. 



7. Drain and dehydrate quickly with absolute alcohol. 



8. Clear in xylol and mount in balsam. 



The Altmann Method: 



1. Stain on slide with Altmann's anilin fuchsin, heating 



once until vapor arises, 6 minutes. 



2. Wash quickly in water. 



3. Differentiate in alcoholic solution of picric acid (made 



by mixing one part of a saturated alcoholic solution 

 with two parts of distilled water) until the sections 

 assume a yellowish-pink color. 



4. Dehydrate very quickly in absolute alcohol, clear in 



xylol and mount in balsam. 



The Benda Method ('01, p. 755) : 



1. 4 per cent, iron alum, 24 hours. 



2. Wash in water, 2 or 3 minutes. 



3. Sulphalizarinate of soda, 24 hours. (Made by adding 



a saturated alcoholic solution of Sulphalizarinate of 

 soda drop by drop to distilled water until an amber 

 color is obtained.) 



4. Dry excess with blotting paper. 



5. Cover with a solution of crystal violet, and warm until 



production of vapor begins, 5 minutes. 

 (Made by mixing 



i vol. saturated solution of crystal violet in 70 per cent, 

 alcohol. 



1 vol. acid alcohol. 



2 vol. anilin water.) 



6. Dry off excess with blotting paper. 



7. Differentiate with 30 per cent, acetic about 3 minutes. 



8. Dehydrate in absolute alcohol quickly. 



9. Pass through xylol to balsam. 



OBSERVATIONS. 



The chief stages in the complicated life history may first be 

 mentioned. 



The vegetative phases or plasmodia are found in damp loca- 

 tions, on the surface of logs, fallen leaves and debris. They are 



