LIFE HISTORY OF AMCEBA PROTEUS LEIDY. 345 



(Fig. 10). These, I was later led to believe, originated from the 

 bodies of the larger multinucleated proteus. Several of these 

 latter individuals while under observation were observed, to give 

 rise to smaller individuals of the form already described. The 

 process was as follows: the animal, which had been moving 

 slowly and apparently without much vigor, gradually came to 

 rest with the pseudopodia upon the then functional anterior 

 portion of the body lobate with slightly pointed tips (Fig. 6). 

 The posterior portion became semi-globular and towards this the 

 greater number of the minute suppositive nuclei within the 

 body plasm migrated. After an interval of about seven to ten 

 minutes the ectoplasm surrounding this globular posterior ex- 

 tremity appeared to disintegrate and from the interior there 

 floated forth several hyaline globules about 4 or 5 microns in 

 diameter. These were followed, after a few minutes by several 

 others, and then a constant outflow began that continued until 

 upwards of thirty of the hyaline spheroids had been extruded 

 (Fig. 7). These were apparently identical with the forms which 

 had made their appearance in the culture. Since these are judged 

 not to be fundamentally different from the pseudopodiospores, 

 of Scheel, but since they exhibited no lobose pseudopodia, they 

 are here called for convenience, apseudopodios pores. Some of 

 these, immediately after extrusion began to move slowly, bulging 

 the very thin ectoplasm at several points, yet without forming 

 any definite pseudopodia. Others, synchronous with their 

 emergence, disintegrated. Still others floated away without 

 exhibiting any signs of motion (Fig. 8). It may be that these 

 were gametic forms, and fused before further development. 



Towards the time of the completion of apseudopodiospore 

 ejection, the parent Amcebce usually gave signs of renewed ac- 

 tivity, elongated perceptibly, and then began apparently to make 

 efforts to move away (Fig. 9). However, after an interval of 

 from twenty minutes to half an hour, they disintegrated. . Some 

 disintegrated at once, leaving the apseudopodiospores behind, 

 but usually they were extruded from the globular posterior 

 portion of the animal, the anterior part retaining its integrity. 

 It appeared as though not all of the minute nuclei were used up 

 at the time of the production of one "litter" of apseudopodio- 



