324 BACTERIA. 



child in the ordinary course of events, Pasteur resolved, 

 after consulting with Professors Vulpian and Grancher, who 

 agreed to share the responsibility, to treat the boy as he had 

 treated the dogs that he had already been successful in pro- 

 tecting. During the following ten days he made thirteen 

 injections : 



2 on the ist day with emulsions made from cords that had been exposed 

 to the air in the flask for 14 and 10 days respectively : 



2 on the 2nd day ,, ,, n and 9 days respectively 



i 3rd day ,, ,, 8 days 



I ,, 4th day ,, 7 days 



and so on until the loth day, when he inoculated with the cord of a rabbit 

 that had died on the same day, i.e., the cord in which the rabic virus still 

 retained its full virulence. 



For every injection that was made into the child, a corre- 

 sponding one was made into a test rabbit, and it was found 

 that the five rabbits inoculated with the first five injecting 

 materials, had no hydrophobia, whilst the other eight 

 succumbed to the disease ; the period at which the animals 

 succumbed being gradually shortened as the cords exposed 

 to the dry air for the shorter times were inoculated. It 

 was remarkable that although the later vaccines proved 

 fatal to rabbits, in the patient, prepared by the previous 

 inoculations, they did not produce the slightest discomfort, 

 he never had the faintest symptom of hydrophobia, and 

 now, five years later, we are told that the boy is still alive 

 and well. Since that time an enormous number of patients 

 have been inoculated, and it certainly appears from statistics, 

 given monthly in the " Annales de 1'Institut Pasteur," that 

 the percentage of deaths after inoculation has been much 

 lower than in those patients left without the anti-rabic 

 treatment. 



Babes, in order to make the virus as constant as possible, 

 finds that it is advisable to make a mixture of cords from three 

 or four different days (z>., cords that have been exposed to the 

 drying process for different periods), and to inoculate at least 

 twice a day, or more frequently in serious cases, such as 

 those in which there are wounds about the face, or where 

 the wounds are inflicted by a mad wolf ; the period of 

 treatment now also is longer, in addition to which much 



