41 6 APPENDIX. 



b. Colonies confluent, growing luxuriantly. 

 (a) Cocci arranged irregularly. 



(j3) Cocci occur as diplococci, or dumb bell shaped organisms, 

 (y) Cocci arranged as sarcinse. 



a. Cocci arranged irregularly. 



(1) Micrococcus candicans forms irregular masses, small yellowish white 

 discs with smooth margins in substance of gelatine ; opalescent or milk 

 white moist flat colonies 2mm. or more in diameter, with indented and 

 sinuous margins at the surface ; dark brown in the centre when seen by 

 transmitted light, but transparent near the thin margin ; nail-head appear- 

 ance in puncture cultivations ; micrococci quite round, moderately large. 



(2) Micrococcus urea. Grows as miliary points like mother-of-pearl, 

 smooth on surface, sharp margins ; these grow rapidly, are well formed 

 in twenty-four hours ; project above surface of gelatine, colony gradually 

 divided by fissures ; along the track of the needle in a tube culture there 

 appear long delicate threads ; there is a large surface growth ; has a peculiar 

 paste-like odour ; grows best at higher temperatures, (30 C.), coccus 0.8 to 

 m in diameter, occurs as diplococci, tetrads, or chains ; along with other 

 organisms, causes decomposition of urea into ammonium carbonate. 



(3) Staphylococcus cereus albusgiw moderately rapidly ; white points on 

 gelatine during the first few days ; in stroke cultivations forms a wax-like 

 layer, with slightly thickened irregular margins along the needle track ; 

 grows on blood serum and potato ; found in pus j usually saprophytic 

 in its action. 



/?. Cocci arranged as diplococci, or dumb-bell shaped organisms. 



(1) Diplococcus lacteus faviformis grows rapidly along track of needle 

 in small points, which run together to form milk white colonies ; found in 

 the sputum and certain secretions as isolated diplococci ; in cultivations 

 occurs as parallel bands of diplococci, each organism being about i.25ju in 

 length and consisting of two hemispheres, between which there is a distinct 

 but narrow fissure. 



(2) Diplococcus albicans amplus. Very like No. I, found in the same 

 positions, but grows in thick white lines along the track of a stroke inocula- 

 tion, organism is comparatively large, measuring 2.25/z in diameter. 



(3) Diplococcus der Pferdepneumonie. Obtained from the lungs of a horse 

 affected with acute pneumonia. Has only been cultivated on gelatine and 

 agar-agar at the temperature of the room ; forms small white, somewhat 

 transparent rounded colonies in agar-agar ; along the line of the needle 

 track in a gelatine tube culture there is seen a row of small white granules, 

 which gradually become larger and coalesce, but there is no special surface 

 growth ; this is an oval micrococcus which sub-divides in its shortest 

 diameter, two of them usually lying together surrounded by a clear homo- 

 geneous capsule ; pathogenic for mice, guinea-pigs, rabbits, and dogs. 



y. Cocci arranged as sarcinse. 



(i) Micrococcus tetragonus forms small white points in gelatine in from 

 twenty-four to twenty-eight hours ; under lens deep colonies have a faint 

 yellow tinge ; mulberry-like surface ; is somewhat raised on the surface of 

 the gelatine along track of needle in puncture inoculation ; first there appear 

 rounded points, which run together ; these grow most readily at surface, 



