STUDIES ON EUGLENAMORPHA HEGNERI. 151 



number of ways. At first it was considered possible that the 

 organisms had come from the surrounding water. Consequently 

 a number of tadpoles were killed by dropping them into Schau- 

 dinn's and Bouin's fixatives. They were then carefully washed in 

 sterile water and examined and found to contain the flagellates. 

 In other cases the tadpoles were opened and the entire digestive 

 tract removed and examined in salt solution without teasing. The 

 flagellates could then be seen swimming actively about in the lumen 

 of the rectum, thus proving their entozoic habit. The hundreds of 

 individuals that were sometimes present in smears made from the 

 rectal contents and fixed and stained also showed that they were 

 obtained from the tadpoles and could not have been derived from 

 an external source. Hegner's ('23) observations also leave no 

 doubt as to the entozoic character of Euglenamorpha. 



For fixation of smears, Schaudinn's fluid has been constantly 

 employed, although occasionally Bouin's fluid and Flemming's both 

 stronger and weaker fluids, chrom-acetic, and sublimate-acetic were 

 resorted to, but with no apparent advantage. For staining, Heid- 

 enhain's iron-alum-haematoxylin has been mostly employed, al- 

 though Delafield's haematoxylin and hsemalum have also been used. 

 The first of these has always given the most satisfactory results. 



All the figures except I to 6 have been drawn with a camera 

 lucida from slides fixed with Schaudinn's fluid and stained with 

 iron-alum-hsematoxylin. Figures i to 6 are drawn from living 

 material on the same scale as the others. All are magnified about 

 1,100 diameters. 



DESCRIPTIONS AND OBSERVATIONS. 



* 



A. Diagnoses. 



Euglenamorpha, new genus. Diagnosis : characters like those of 

 Euglcna except for the three to six flagella and the entozoic habit. 

 The Euglena-like. characters include elongated body, presence of 

 chlorophyll and red stigma, vacuolar and pharyngeal apparatus, 

 nucleus with central caryosome surrounded by chromatin in the 

 form of granules or strands, periplast spirally striate, metabolic 

 activity. The distinguishing characters of the new genus are (i) 

 the presence of three to six flagella and (2) the entozoic habit. 



