STUDIES OX EUGLEXAMORPHA HEGXERI. 155 



10.5 per cent., had five; and 79, or 39.5 per cent., had six. Indi- 

 viduals with two or three flagella (Figs. 36 and 26) may be daugh- 

 ters from a recent division. The significance of the four- and six- 

 flagellated condition is problematical, but will be discussed further 

 on. The two groups differ in size, it was found. A series of 

 four-flagellated individuals were found to range from 32 to 44 

 microns in length, with an average of 38.25 microns, while a series 

 of six-flagellated individuals averaged 45.6 microns in length, with 

 a range of 42 to 52 microns. The roots of the four or six flagella 

 are usually segregated into two equal groups, with the correspond- 

 ing groups of basal granules some distance apart in the posterior 

 wall of the reservoir. This condition is probably correlated with 

 the prevalent stages of amitosis found in the nucleus (Figs. 12 to 

 16 and 32 to 35). 



The reservoir is regularly of larger size in pclludda than in the 

 type and the absence of other stainable material makes it easier to 

 distinguish the basal granules of the flagella in its posterior wall. 

 No rhizostyles extending from the basal granules toward the 

 nucleus have been found. 



The stigma (s, Fig. i), found only in the green variety, has a 

 structure similar to that of other Euglenoids, being a disk of closely 

 aggregated granules placed peripherally in the region of the reser- 

 voir adjacent to the swellings on the roots of the flagella. These 

 swellings and the stigma probably have some coordinate function 

 and usually disappear simultaneously in the transformation to pcl- 

 ludda. The stigma does not persist in slides fixed and stained by 

 the usual processes, but did persist for a day or two in smears 

 fixed with osmic acid fumes and mounted directly in glycerine jelly. 



Another difference between the two varieties is revealed in the 

 cultures where the type variety persisted much more readily than 

 did the colorless one. In certain cultures the green variety went 

 into a resting state where it divided (Figs. 4 to 6). In other cul- 

 tures they multiplied in the active state. The pellucid variety 

 showed neither of these conditions. 



In the abstract already published (Wenrich, '23) it was stated 

 that division stages had not been seen for the green variety except 

 in cultures, and that this fact constituted one of the differences 

 between the two varieties, both mitosis and amitosis having been 



