158 I>- H. WENRICH. 



disappeared and an extra flagellar root without a swelling shows 

 in the reservoir. The origin of the four-flagellated pelludda is 

 possibly indicated. 



In one of the cultures (see below) a pellucid individual appeared 

 among the green ones a month after the culture was started. It is 

 probable that it transformed from a green one. Altogether the 

 evidence at hand points to the occasional transformation of the 

 green variety into the colorless one. 



C. Results from Cultures. 



In attempts to cultivate Euglenamorpha outside the host two 

 general methods were employed. In one a section of the rectum 

 or a piece of its contents bearing the flagellates was placed in a 

 small stender dish containing the medium. The other method was 

 that of the hanging drop, in which a drop of the medium was 

 placed in the middle of the cover glass, the rectal material added, 

 and the cover then inverted over a depression slide and sealed with 

 vaseline. 



In the fifteen dish cultures the following media were tried : ovo- 

 mucoid, beef bouillon, Sellard's ('n) liquid medium modified by 

 Dr. Martha Bunting ('22), who very kindly prepared the fore- 

 going media; Pringsheim's ('15) solution, Ringer's solution, 0.6 

 per cent, sodium chloride, equal parts of i per cent, sodium chlo- 

 ride and i per cent, sodium citrate, sterile pond water, various com- 

 binations of these and the use of some of them in combination with 

 2 per cent. agar. In the eleven hanging drop cultures the above- 

 named media were also used except the ovo-mucoid, the beef 

 bouillon, and the agar. 



None of the dish cultures served to keep Euglenamorpha alive 

 more than a few days and in none of them was there any evidence 

 of multiplication. Tricliomouas flourished on a mixture of ovo- 

 mucoid and 0.6 per cent. NaCl, and the desmids and Phacus did 

 well in Pringsheim's solution. 



The hanging drop cultures were, in general, more successful. 

 Culture F, a drop of Sellard's modified liquid, was interesting be- 

 cause of the changes which took place. The flagellates (green 

 variety) went into resting state (Fig. 4) during the first day, but 

 began to resume activity at the end of a week. Some divided in 



