STUDIES ON EUGLENAMORPHA HEGNERI. 159 



the resting state (Figs. 5 and 6). On resuming activity they first 

 put forth a single flagellum and began to swim about with it, later 

 two more flagella emerged. In some individuals the chlorophyll 

 became unevenly distributed, being concentrated at either end. In 

 others the chlorophyll disappeared entirely, but such colorless indi- 

 viduals retained the form of the green variety. Various species of 

 Euglena, Phacits, and desmids multiplied in this culture and Eu- 

 glenamorpJia persisted until transplants were made at the end of 

 a month. 



In culture K (0.6 per cent. NaCl) there were at the start two 

 or three dozen of the green variety and half dozen pellucid a. At 

 the end of five days all the colorless individuals were dead, but the 

 type variety had begun to multiply. At the end of three weeks 

 there were between 300 and 400 of the green individuals. The 

 numbers remained stationary for a few days, then gradually de- 

 clined, but a few survivors were still active after five months. 

 After the first few weeks, many of the flagellates moved to the 

 edge of the drop nearest the window, remained stationary except 

 for the lashing of the flagella for varying lengths of time, and then 

 began to disintegrate. In the disintegrating individuals the para- 

 mylon granules often persisted apparently unchanged for days or 

 weeks, while the remainder of the body completely decomposed. 

 Very, few entered the resting stage such as shown in Fig. 4. 



Culture L was made with the sodium chloride-sodium citrate 

 mixture. During the first five days not more than one of the 

 green variety was visible, but during the following two or three 

 days the number increased to nearly three dozen. When the cul- 

 ture was a week old a binucleate Opalina appeared, having prob- 

 ably emerged from a cyst. Multiplication followed till there were 

 sixteen Opalina at the end of the five more days. The Opalina 

 and Euglenamorpha were then transplanted to new hanging drops, 

 but did not survive. 



In culture O (Pringsheim's solution) there were between one 

 and two dozen of the green variety at the start. At the end of a 

 week no active individuals could be found, the flagellates having 

 either died or assumed the resting state. At the end of another 

 week several resumed their active condition and remained active 



