166 D. H. WENRICH. 



Division of the green variety in the host is rare, but appears to be 

 accompanied by transformation which may also take place without 

 division. Once established, the colorless variety may multiply rap- 

 idly by both mitosis and amitosis and consequently outnumber the 

 green one. 



7. Attempts to culture the flagellates outside the host were more 

 successful with hanging drops than with dishes. The most suc- 

 cessful culture was a hanging drop of 0.6 per cent. NaCl in which 

 the green variety multiplied for three weeks, then gradually de- 

 clined, but some few were still alive after five months. In other 

 cultures a few appeared to transform from the green to the color- 

 less variety. 



8. The movements of E. hcgncri, both swimming and metabolic, 

 may be very rapid. In some cases the organisms appear to attach 

 themselves to the wall of the rectum or other object by one flagel- 

 lum and to vibrate the others near the body, thus possibly serving 

 both a respiratory and a nutritive function. They are positively 

 phototropic. 



9. Outside the host (in culture) the green variety may assume 

 a resting state approximating encystment in which it may divide 

 and by means of which, presumably, it reaches a new host. The 

 colorless variety is not known to assume the resting state and 

 appears to be too unstable to be a permanent form. 



10. One is tempted to regard the entire situation as revealing an 

 uncompleted series of stages in the evolution of a colorless parasite 

 from a free-living, green euglenoid flagellate, but the evidence is 

 not entirely conclusive. 



REFERENCES CITED. 

 Alexeieff, A. 



'15 Le parasitisme des Eugleniens et la phylogene des Sporozoires, sensu 

 strictu. Arch, de Zool., Exp. et Gen. Ser. 5, T. 10. 



Beauchamp, P. de. 



'n Astasia captiva, n. sp. Euglenien parasite de Catenula lemn<r Ant. 

 Dug. Arch, de Zool., Exp. et Gen. Ser. 5, T. 6. 



Bunting, Martha. 



'22 A Preliminary Note on Tetramitus, a stage in the life-cycle of a copro- 

 zoic amoeba. Proc. Nat. Acad. of Sci., Vol. 8. 



Haswell, W. A. 



'92 Note on the Occurrence of a Flagellate Infusorian as an Intracellular 



Parasite. Proc. Linn. Soc., N. S. W., ad ser., Vol. 7. 

 '07 Parasitic Euglenje, Zool. Anz. Bd. 31. 



