I6O ]> H. WENRICH. 



with no apparent multiplication for two or three weeks longer. 

 Eugleiia and Phacus multiplied readily in this culture. 



Culture P was made with equal parts of modified Sellard's 

 liquid and Pringsheim's solutions. During the first three days the 

 numbers increased from about three dozen to over a hundred, but 

 some had already gone into the resting state or begun to disinte- 

 grate. Nothing but the green variety could be seen. During the 

 following week the numbers of active individuals gradually de- 

 creased and then an individual appeared with the form and activity 

 characteristic of pellucida, but a slight amount of color was still 

 discernible. Two weeks later several individuals showed the form 

 and behavior of pellucida and in one the four flagella could be 

 made out. Transformation seems to have occurred in this case. 



Culture R, like K, was made with a drop of 0.6 per cent. NaCl. 

 Only the green variety appeared at first. There was rapid multi- 

 plication for three days followed by a rapid decline of the culture. 

 Some individuals lost all color, but retained the form of the green 

 variety. 



Attempts to transplant the organisms to new hanging drops or 

 to dishes were frequently made, but in none of these subcultures 

 did Euglenamorpha survive more than a day or two. 



Of the total of twenty-six original cultures made, only a few of 

 the hanging drops could be considered successful. These few did 

 demonstrate, however, that the green variety can be cultivated out- 

 side the host, that outside the host a resting state may be assumed 

 during which division may occur, and by means of which, pre- 

 sumably, access to new hosts could be obtained, and that the green 

 variety may become colorless and probably change to pellucida. 



E. Behavior. 



One of the striking features of these flagellates is their great 

 activity. No other euglenoid that the writer has ever seen can 

 equal Euglenamorpha in rapidity of movement of either the swim- 

 ming or metabolic (" euglenoid ") type. Examined in the rectum 

 of the host, they can be seen swimming rapidly about or stationary 

 in contact with the wall of the rectum or its contents. When 

 teased out into salt solution or water they usually increase their 

 activity, adding the " euglenoid " form changes to the swimming 



