SECRETION IN FOLLICLE CELLS. 



217 



state at the time of implantation were drawn with camera 

 lucida and observed from time to time for periods of which the 

 maximum was five days; but no significant change in shape of 

 either nucleus or nucleolus was noted in either stained or un- 

 stained cultures, although the procedure was repeated many 

 times. It is possible that the nuclear strands may have changed 

 slightly in relative position, but such a change was difficult to 

 observe with accuracy. The cells of the D and younger follicles 

 were found too small to be observed satisfactorily. 



When it was ascertained that division could not be seen taking 

 place in the cells of the A and B follicles, forty follicles of the 

 large cricket were fixed and sectioned. Of this group, ten A 

 follicles were sectioned longitudinally and ten transversely; and 

 the number of cells in the largest section of each longitudinally- 

 cut follicle was counted, as well as the number of cells in the 

 largest section of each transversely-cut follicle. The same 

 procedure was carried out for twenty B follicles. Results follow: 



From the foregoing tables it appears that while there is 

 considerable variation among individual follicles of each class as 

 to their number of cells, the average number in the B follicle is 

 similar to the average number of cells in the A follicle, although 

 there is great difference in size between A and B. This indicates 

 that during these two stages very little, if any, increase in the 

 number of cells in the follicle occurs. Moreover, nearly all of 



