45 ELERY ROXALD BECKER. 



as they apparently do in the form from A. Umax. Third, there 

 is no nucleus present within the spores of S. endamcebx, although 

 the contents of the spore are clearly visible. Chatton and 

 Brodsky state that it was difficult to see the interior of the 

 spores of their Sphxrita even in stained specimens. In a few 

 cases, however, they observed an excentric nucleus within the 

 spore. Fourth, the appearances of dividing nuclei of the multi- 

 nucleate plasmodium dffer in the two species. Those of 5. 

 endamosbx are bilobed, or dumb-bell shaped. Those described by 

 Chatton and Brodsky presented the appearance of two cuneiform 

 polar caps. Measurements of the two species in various stages 

 lie within approximately the same limits. The above stated 

 facts make it evident that the Sphxrita of the Endamcebx is 

 altogether different from that of the free-living amoeba, A. Umax. 



Likewise I believe it is different from the one figured by 

 Dobell (1919) in Endolimax nana, an amoeba entozoic in man. 

 If his figures be correct, the nucleus of the uninucleate stage 

 is punctiform and excentric, as in the parasite of A. Umax. The 

 morula-shaped mass of spores is likewise not characteristic of 

 S. endamcebx. Kessel's (1924) account is too meagre to afford a 

 comparison. 



Chatton and Jansky were not able to determine whether the 

 spores of their Sphxrita became flagellated, after the manner of 

 the zoospores of Sphxrita endogena and S. dangeardi as described 

 in the accounts of Dangeard, or remained immobile and were 

 passively ingested by the amceba. Although actual reinfection 

 by the spores was not observed, the writer has been able to follow 

 out the process in his prepared slides. 



Among the bacteria present on the slide one occasionally finds 

 dumb-bell shaped organisms, resembling Azotobacter. There 

 seem to be two general sizes, one considerably larger than the 

 other (Fig. 10). These multiply by binary fission (Fig. n). It is 

 not unusual to find these dumb-bell-shaped bacteria-like bodies in 

 the food vacuoles of the amoebae (Figs. 8, 12, 13). In the food 

 vacuoles the organism undergoes considerable change. Dense 

 granulation appears in the more or less homogeneous cytoplasm. 

 The dark granules collect in a deeply-staining clump in the center 

 of the cell (Figs. 3, 8, 14). The two members of the dumb-bell 

 shaped pair usually separate, and are carried some distance from 



