JAMES ERNEST KINDRED. 



spherules; d, amebocytes with acidophilic spherules; and e, 

 amebocytes with green spherules. Kollmann (1908), using the 

 triacid stain of Ehrlich, following fixation with Zenker's fluid, 

 does not find leucocytes with neutrophilic granules. Of the 

 amebocytes with spherules, he studied only those with basophilic 

 spherules. He states that these spherules are amphophilic. On 

 comparing the tinctorial reactions of the several types of cells 

 with their characters in the living condition, St. Hilaire interprets 

 the basophilic spherules as identical with the colorless spherules 

 of the living cell, the acidophilic as identical with the reds, and 

 the green spherules as degenerate reds. 



According to Kollmann the chemical composition of the color- 

 less spherules is complex. They contain an albuminoid substance 

 insoluble in alcohol and an alcohol-soluble lecithin. The red 

 spherules seem to be of a more complex albuminoid nature and 

 also contain a lecithin. The albuminoid nature of the spherules 

 of those cells with colorless spherules accounts for their relatively 

 slow rate of movement and also for their greater number in the 

 connective tissue as contrasted with their number in the peri- 

 visceral fluid. Since the mast cells of vertebrates exhibit com- 

 parable tinctorial reactions and connective tissue affinities, 

 Kollmann infers that the amebocytes with colorless spherules are 

 comparable with them. 



Kollmann also states that although he can find no relation 

 between leucocytes and amebocytes with spherules, it is possible 

 that the latter arise from the former by the development of 

 spherules within the hyaline cytoplasm. 



The following investigation has been undertaken to determine 

 a possible source of origin for the amebocytes with spherules 

 from leucocytes. The inspiration for this investigation came 

 from the suggestion by Cuenot ('91) that the spherules are 

 extrinsic in origin, nutritive in nature, and that the amebocytes 

 with spherules may arise from leucocytes which have ingested 



food droplets. 



MATERIAL AND METHODS. 



Examinations were made of fresh drops and fixed smears of 

 perivisceral fluid of Arbacia punctatum. Some of the smears 

 were fixed in Hellv's fluid and stained either with the Giemsa 



