224 MARGARET R. MURRAY. 



region of some of these cells there has been seen a nebulous 

 structure composed of very finely divided black particles, which 

 might be considered to indicate an excessive deposition of fat in 

 the tissue. This fine emulsion might conceivably be coarsened 

 at the boundary of the cell, and coalesce into fatty globules. 

 (See Fig. 5.) 



What part the egg itself may play in the synthesis of these 

 yolky materials has not been ascertained. It is remarkable that 

 no substances which could be identified as Golgi bodies have 

 been found in either follicle cell or egg, although several standard 

 fixatives for Golgi bodies have been used, and many kinds of 

 inclusions preserved. With the use of either the Champy or 

 the Kopsch preparation alone, doubtless the distal droplets 

 would have been considered to be Golgi bodies. But all the 

 observations combined make such a diagnosis doubtful for these 

 or any other observed inclusions, in either egg or follicle. 



V. DISCUSSION. 



A. SIGNIFICANCE OF NUCLEAR CONSTRICTION. 



A general discussion of the significance of amitosis and a 

 review of the literature on the subject will not be attempted 

 here in view of the full surveys afforded by Conklin (1917), 

 Xakahara (1918), and Bast (1921). 



Study of cell division in the "amitotic" follicle cells of the 

 cricket has shown that in this form the division of the nucleolus 

 and constriction of the nucleus rarely if ever are followed by 

 the constriction and division of the cytoplasm; it has also shown 

 that complete cell division is not necessary to account for the 

 increase in the size of the follicle between the B and the A stages. 

 That the amitotic behavior of the nucleus here is an indication 

 or result of senescence seems open to question in view of the 

 fact that at this time the follicle cells are metabolizing at a 

 high rate in the elaboration of materials which go to make up 

 the yolk of the egg, and therefore cannot, so far as metabolism 

 is concerned, be called senescent. They are, it is true, highly 

 differentiated cells, and in that sense the term senescent may be 

 applied to them. But it seems to the writer that this amitotic 

 behavior, confined as it is to the nucleus, should be given a 



