PROTOZOA AND THE UTRICLKS OF UTRICULARIA. 26l 



had occurred within; others become opaque and abnormal in 

 shape and then begin to break down at one or two points usually 

 on the side; and still others although opaque and abnormal in 



shape retain their identity. 



/CQ ~ 



Experiment 52. The paramecia in this experiment disintegrated into a mass of 



jtiJ I- I 

 granules in the attached bladders that had been inoculated with them within 26 



minutes, 24 hours, 2 days, 4 days and 5 days respectively. 



Experiment 53. The paramecia became granular in attached bladders that had 

 captured them within the following number of minutes: 38, 68, 70, 83, 85, 123, 

 124, 127, 194. In at least 9 bladders they remained intact for 18 hours. 



Experiment 54. The paramecia became granular in attached bladders that had 

 captured them within the following number of minutes: 27, 70, 71, 125. Five 

 became granular within 2\ hours, and 8 within 20 hours. 



Experiment 55. Two specimens became granular in attached bladders that had 

 captured them within 2 hours, 2 within 2\ hours, and 12 within 2\ hours. Fifty- 

 five were still intact at the end of 2\ hours. 



Experiment 57. The paramecia became granular in attached bladders that had 

 been inoculated with them as follows: in 10 bladders within 18 hours; in 16 

 bladders within 24 hours; and in 8 within 48 hours. 



Experiment 56. The paramecia became granular in detached bladders that had 

 captured them as follows: 6 within 40 to 58 minutes; 12 within 64 to 95 minutes; 

 and 2 within 2\ hours. One was still alive and 143 were dead but not granular at 

 the end of 4 hours. 



Experiment 5#. Paramecia in this experiment were captured by detached irri- 

 gated bladders. Seven hours later 3 specimens had become granular, n were 

 dead but not yet broken down and one was still active. 



Experiment M. 13. Paramecia were inoculated into attached irrigated bladders. 

 No accurate record was kept but many specimens were granular at the end of 

 5 hours. 



This series of experiments indicate that paramecia break down 

 into a mass of granules under various conditions, (i) in attached 

 bladders that have captured them, (2) in detached bladders 

 that have captured them, (3) in attached bladders inoculated 

 with them, (4) in attached irrigated bladders inoculated with 

 them, and (5) in detached irrigated bladders that have captured 

 them. The principal differences seem to be the length of time 

 required to reach the granular state and the comparative number 

 that become granular within a brief period, e.g., within one hour 

 or 2\ hours. A larger number of paramecia became granular in 

 a shorter length of time within bladders that were attached or 

 detached and had captured them (Exps. 53, 54, 55, 56) than in 

 bladders into which they had been inoculated. No differences 

 are apparent between attached and detached bladders. From 



