376 



L. J. BLACHER. 



red spots were almost invisible. Also the black spots found on 

 the side of the body lost their intensity. The specimen died on 

 May 24, 1925. A very slightly developed testis was found after 

 examination of the viscera. (Figs. 2 and 2a.) 



5. Male Fish No. 33. The specimen was born in August, 1924, 

 and reached maturity with secondary sex-characters well formed. 

 From May, 1925, on, the characteristic male shape was under- 



FIGS. 1-5. 



going a change toward that of the female. This was closely 

 followed by the disappearance of sex colorations. Figs. 3 and 30. 

 On June 4, 1925, it was killed and preserved in formol-alcohol 

 solution. Sections eight microns thick were cut and stained in 

 hematoxylin. Upon examination it was found that the testis 

 was very small and almost deprived of spermatocysts. It con- 

 tained neither primordial germ cells nor spermatocytes. Sper- 

 matogenesis was encountered here and there but only the later 

 stages. The connective tissue of the gonad was well developed. 



