246 A. M. FIELDE. 



the two united sections, again without young, another year, until 

 August, 1904, when I introduced to their nest marked queens 

 and workers from their old wild nest. Of the two queens intro- 

 duced one was at once received into full fellowship. The other 

 was simultaneously licked and dragged by different worker- 

 residents, but was accepted at the pupa-pile within a few hours. 

 The workers introduced had kindly reception. Of one hundred 

 and fourteen callows introduced one by one, or a few at a time, 

 an interval of repose being given between the removal of one and 

 the introduction of its successor, only two were attacked. While 

 it is true that all the accepted callows of the summer of 1904 

 might have had the odor of the resident queen, it appears more 

 probable that most of them bore odors that were recognised by tlie 

 resident ivorkers after the lapse of three years. 



These ants had certainly not within their three years of segre- 

 gation met a two-year-old or a one-year-old ant of their colony, 

 from outside their artificial nest. In August, 1902, I segregated 

 a queen and workers all hatched during that month, and in 

 August, 1903, I likewise segregated a newly hatched and similar 

 group. All these ants were of the C colony, and no young was 

 permitted to hatch in any of these groups, each in its artificial 

 nest. In August, 1904, I therefore had command of ants of the 

 C colony, one group in the C nest, consisting of ants brought in 

 from the wild nest on June 24, 1904; one group Ci, just one 

 year old, one group C2, just two years old, and one group 3, 

 three years old or more. All had been acquainted with their 

 seniors before segregation. In August, 1904, the three-year-old 

 ants received amiably, within their nest, ten of the two-year-old 

 ants, and ten of the one-year-old ants, indicating a perfect recog- 

 nition of odors no longer represented in their own nest, and from 

 which they had been long separated. 



On the other hand, when I introduced the three-year-old ants, 

 queens or workers, into a nest populated with hundreds of 

 workers taken in June, 1904, from the wild nest or hatched 

 within that nest during the present summer, the three-year-olds 

 were always fiercely attacked. Tlicy had become an alien colony 

 to the younger generations of their former wild nest. The C col- 

 ony has been much harried in its natural domain, by the rebuilding 



