I3O C. M. CHILD. 



Scries 



November 21, '02. - Ten large species of C. solitaries were 

 cut transversely a short distance aboral to the oesophagus (Fig. 

 i ), the portions aboral to the cut being used for experiment. In 

 five of these pieces the body was split longitudinally for half its 

 length from the aboral end and oblique incisions were made at 

 various points on the longitudinal cut surfaces (see dotted lines 

 in Fig. i). The object of this procedure was to produce great 

 irregularity in the cut surfaces at the aboral end in order that the 

 inrolled edges might not fit closely together but leave numerous 

 spaces between them, thus permitting communication between 

 the enteron and the exterior. These five pieces may be desig- 

 nated as the experimental pieces or the experiment. 



The remaining five pieces were not injured aborally ; they 

 were placed in the aquarium under the same conditions as the 

 first five and remained undisturbed, serving as a control. 



From the beginning of the experiment to December 8 the 

 experimental pieces were reopened on alternate days, /. e., the 

 slime was cleaned from the aboral ends and with the aid of 

 needles and forceps the end was spread widely open, care being 

 taken in each case to ascertain that the communication between 

 the enteron and the exterior was unobstructed. From Decem- 

 ber 9 to December 17 they were reopened daily. The terms 

 employed in describing the various stages are explained by 

 reference to the first paper of the series (Child 'o$a). 



November 28. 7 days after section. Experimental pieces; 

 no tentacles present ; tentacular ridge not distinct ; oral end not 

 expanded. 



Controls : one piece distended with tentacular ridge appear- 

 ing ; other pieces partly filled ; tentacular ridge not distinct. 



December 2. - - \ \ days after section. Experimental pieces : 

 A few minute tentacle-buds on two pieces ; two others with 

 tentacular ridge just appearing. All four of these pieces have 

 succeeded in closing aborally more or less perfectly and becom- 

 ing more or less distended in the intervals between the reopening 

 of the aboral end ; the fifth piece remained widely open aborally 

 and shows no trace of tentacles. 



Controls : All with distinct tentacular ridge and some tentacle- 

 buds just appearing. 



