248 A. M. FIELDE. 



years old, and that of a queen. I then, a day or two later, in- 

 troduced into its cell, one by one, all the one-year-old ants in 

 nest Ci, removing each visitor as soon as the action of the resi- 

 dent was decisive, and allowing a period of repose before another 

 visitor was introduced. She affiliated with the first, third, fourth, 

 sixth, seventh, eighth, tenth and eleventh, and attacked the sec- 

 ond, fifth and ninth. 



I likewise introduced two-year-old workers from nest C2. 

 She affiliated with the first, third, fourth, fifth, seventh, ninth and 

 twelfth, and attacked the second, sixth, eighth, tenth and eleventh. 

 She did not attack the C2 queen, but the queen so persistently 

 avoided her as to make the test undecisive. 



In the same manner I tested a callow, hatched on July II, in 

 nest C2, where all the ants beside herself were two years old. 

 When this callow had spent seven days in the C2 nest, and one 

 day in isolation with larvae to care for, I introduced into her cell 

 workers from the C3 nest, where all the ants were three or more 

 years old. She affiliated with the second, fourth, fifth, eighth, 

 eleventh and twelfth, and attacked the first, third, sixth, seventh, 

 ninth and tenth visitors. I then introduced one-year-old ants 

 from nest Ci. She affiliated with the first, second, third, sev- 

 enth, eighth, ninth, tenth and twelfth, and attacked the fourth, 

 fifth, sixth and eleventh visitors. 



I intended to likewise test a callow reared in nest Ci, and I 

 expected to find that this callow would reject all three-year-old 

 ants ; but I unfortunately dropped an unmarked three-year-old 

 ant into nest Ci, and thereby so vitiated the nest as to make it 

 useless for this experiment. 



A comparison of all the tests made gave a consensus of testi- 

 mony that the C3 ants, the Stenanunas, recognized and adapted 

 their beliavior to ant-odors that they had not encountered during 

 three years. 



As the workers are not supposed to reproduce colonies, and 

 as the queens are not supposed to change their own odor, how 

 then would queens of diverse odor originate through the ageing 

 of the workers ? 



In 1901 I segregated winged queens of the C colony, 1 putting 



1 " Notes on an Ant," previously referred to, p. 605. 



