I4O C. M. CHILD. 



September 22. 6 days after section. None of the pieces 

 closed and none showed any tentacle-buds. On ordinary 

 cylindrical pieces regenerating tentacles appear in about four to 

 five days at this time of year and are usually I mm. long on the 

 sixth day. 



September 28. 12 days after section. Four pieces possessed 

 minute tentacle-buds on at least a part of the oral end. The 

 pieces were variously rolled, those with tentacles at least in part 

 longitudinally, the other four in part transversely. All were 

 more or less widely open in the middle region. Two of the 

 pieces at this stage are shown in Figs. 12 and 13. In Fig. 12 

 the portion on the right has rolled longitudinally in a spiral and 

 at the oral ends the opposing margins of the spirally rolled por- 

 tion are united by new tissue and bear minute tentacles. The 

 remaining portion of the oral end is rolled aborally behind the 

 tentacle-bearing portion, much as in Fig. 10. 



In Fig. 13 the inrolling is almost wholly longitudinal and at 

 the oral end new tissue has united the whole circumference. 

 The longitudinal cut margins are rolled inward, but not united, 

 though closely approximated. The circle of tentacle-buds is 

 almost complete. 



October 5. - - 18 days after section. Six of the pieces are 

 rolled transversely in a spiral, having changed somewhat in form 

 since the last examination. In one of these in which the transverse 

 inrolling began at the aboral end a few tentacles 2 mm. in length 

 are present ; in the other pieces there was no change as regards 

 tentacles. The elongation of tentacles in this one piece was ap- 

 parently due to the accumulation of some water in the outer 

 turn of the spiral, i. e., the more oral part of the piece, the mar- 

 gins of this turn being applied to the next inner turn closely 

 enough to permit the establishment of some degree of water- 

 pressure. 



A few days later all eight of the pieces were dead, probably 

 on account of accidental insufficiency in the water-supply. 



Scries 28. 



September 26. Nine specimens were cut transversely aboral 

 to the oesophagus, then the aboral end was removed and the pieces 



