CHEMICAL EQUILIBRIA 119 



experiments of MADSEN and myself regarding the 

 haemolytic action of mixtures of NH 3 with H 3 O 3 B, 

 LUNDEN measured it according to the methods used 

 in physical chemistry and found it to be K = i -04 

 at the same temperature (37 C.), which is an ex- 

 tremely good control on the validity of the theory 

 adopted. 



According to the same method and formula I 

 calculated the figures of MADSEN for tetanolysin 

 and found K = 0-115 at 37 C. At i6C. it is 1-91 

 times smaller, from which we may calculate that at 

 the binding of one gram-molecule tetanolysin to 

 one gram-molecule antilysin there are formed two 

 molecules of their compound with an evolution of 

 5480 calories. The curve representing the neutral- 

 ization falls between that characteristic for ammonia 

 with boracic acid, and that for diphtheria toxin with 

 its antiserum. The agreement between observation 

 and calculation is very good and wholly within the 

 errors of observation (see Fig. 30, middle curve, 

 and the following table). 



[TABLE 



