the undigc-ted lihriu. C < unpare the color of the hinrei tost given hy 

 tuhc- li t" e. Kc-ult? I'onclu-ion- as in condition- of activity 

 ..fpepsin? Does 0.29! I U'l alone effect hydrolysis of proteid? Xote 



cially difference in appearance oi a and 1. 



l:.vtimiiiatiini f pcpsin-HCl tlit/cst. Allow a solution 

 gg white to digest overnight with pcpsin-IK'1. Then 

 :inine tin- digc-l as foll"\\ - : A. Xcutrali/e; i- there any precipi- 

 \\hat i- tin- precipitate ? I'.. filter; to a part of filtrate 

 appl\ ferrocyanide or picric acid tc-t ; re-ult? Conclusion? C. Satu- 

 rate the remainder of the filtrate from A with (XII,),S<>, and 

 filter; apph the hiuret U st to a portion of the filtrate; re-ult? Con- 

 clusion? Add hr mine water to the remainder of the filtrate. 

 Am coloration? 



I'oc- (/(/</ alone digest proleid? lloil a few thin -fip- of egg- 

 white for s,>nie time (at lea-t jo minute-) in \a' , lll'l. Coo] and 

 apply the hiuret test; re-ult? 1 )oe- pepsin digest proteins in the 

 ah-encr of free acid ? 



'/>Y/\\-;;; an enx\me occuring in pancreatic juice and active 

 in alkaline solnti n. Use a -olution of tryp-in in \' , XaA'<'. 

 Taki 5 lr-t-tnhr- and to each add a -trip of fihrin. I hen add: 

 a. 5 or ID cc. l ' , Xa_.l '< i . 

 1). $ OT i< ' CC. t r\ ji-in -i >lutii m. 



The -ame. e\actl\ neutralized with \' , IIC'l, using i drop 

 of neutral red a- an indicator. 



d. The -ame a- c -(- ecjiial x'olur.ie of o._>', 11(1. 



e. 5 or KI cc. trvpsin solution previoiish' hojled. 



I'lace in the water hath at } I '.. and oh-er\'e at intervals to -ee 

 it dige-tion occurs. After |o min. remo\e the undigested lihrin and 

 tor peptone with hiuret reagent. Xote especially the color of 

 the hinret n-ai'tion in each case. In uhich i- peptone formed? 



niiniliini /if tryptu' <//</<>/. Let 5 cc. egg-while digest 

 "\'Tiiight witli ]iancrea- extract. Lxaminc as follows: 



Lilh r the digest. Saturate a ]iorti..n of the fillrate with 

 ammonium sulphate; filter and te-l the fillrate for peptone. 



To another porti, >n of the filtered di-e-t add gradually hroniinc 

 Xote tin ensuing coloration. ( 'oinpare with gastric digest. 

 ( V'-v/ 1 /. ///(///, i. -t. i 



tration of amino acids i Icucin and tyrosin). 1 ; .\ aporale 



the remainder . .l" the original filtrate to the consistency of a -yrup. 



al'ohol gradually t<> tin- s\ rnp\ solution until no further 



te form-. >tirrin- co;itinuall\ \\ith a gla-s md. \\'hat 



