tratioiis <>f the-c -nit-lances in the -olmion. I.ipa-c is the mo-t 

 fa\. .ral.le en/yme for tin.- <K monstrati> >n of this property. Take 



a iv u-e tlu- exact amount- called f< >r in the directions, 

 a. //V(/r'/v/. . r -plating, action of lipa-e. I .ahel _' -mall tla-ks 

 I ul. es a am! h. In a place i<> CC. of neutral pancreatic ex- 



I etlnl butyrate u'l ! < I I t I I..O H U'JL). 



k tiidith and keep at )" for 4" minute-. Kemove to ice \\ater 



until quite cold. ('..].. r with litmu-. \\liat is reaction? Xow empty 



the content- into a heaker ami titrate iniinciinitcly with n jo \a( >l 1. 



i.e. ad-1 the alkali carefully from the hurette until the color ju-t 



change- to blue. N'ote a- exactly a- |>o--iMe the amount of alkali 



lla-k h aM the -ame (|uantitie- of ethyl hutyrate 



and |>ancreatic juice f'rcriottsly />;7r</ and proceed in the -ame man- 



( -..npare the re-ult- of a and h. Write the equation for the 



-plittini,' of ethyl hutyrate: of glycerol hutyrate ihuttcr fat). 



h. Synthetic nction <>) Lipusi-. Mix _'5 cc. of m _'O hutyric acid 



and 10 CC. - '' , ethxl alcohol. I'lace _'() cc. in each of two flasks, a 



and h ; to a add 5 cc. pancreatic extract; to h 5 cc. pancreatic 



i xtracl previously h<>iled. ( '<>rk hoth tla-k- tightly and place in a 



water hath at .\ for J<> minutes. 'Then compare the dr from 



the two tla-k-. 1- the ethyl hutyrate to he detected? In which 



I low forim-d? \\rite the e<|uali'.n. l ; .ni|>tv the contents ot 



li tla-k into a heaker and titrate with n Jo K()ll. usiiij^ litmti- 



aii indicat'ir. Mow much i- required for each tla-k? 1 )o your 



'.It- indicate that -ome acid ha- disappeared through -ynthe-i- to 



!' inn the ester? 



i' i. Influence ./ temperature an enzyme action. ( (Jimntita- 



tleteniiiiiiitioii. i I'ollect -aliva, dilute with ~ volumes of water 



and t'dter. \d<l ; cc. <lilutc- starch paste to each of four test-mite-. 



"lie t< -t tnhe in the \\ater hath at 40 '.. and keep another at 



in temperature i ahont ji t ('. ). Have two series of iodine drops 



"n a porcelain plate. When the te-t tuhe- have attained the 



j-p.per temperature- add an equal volume of dilute saliva to each 



tnhe. mix thoroughly, and keep at their respective temperature -. 



At inteixal- iif niie half minute remove a drop ,>f the mixture and 



h. < 'ne -indent attend to the le-l- from 4C -ali\a 



in: the other to the 20 mixture. Determine the time required 



convert the March into pruduct- L;ivint, r no colur reaction \\ilh 



ich temperaf 



.ictl\ the -ame experiment n-iiii; the Siiinc diluted 



\-"' hut I ..... it o C and compare it with another 



