(if water to serve a> indicator of the rate of gas flow. All junc- 

 tions must he rendered gas-tight. Using paraffin where neee--ary. 



Vote the effects of lack of oxygen ( 1 ) on the activity of the 

 ulia and contractile vaciiok-- ic.ni]>are at interval with the "con- 

 trol", i.e.. Paraniecia in normal culture fluid; ( _' i on the cou-i>- 

 tcncy of the protoplasm ; 1^1 on the absorption of water 1>y the 



I >etennine the degree of reversibility of the effects. After the 

 movement- have alnio>t ceased. expos^ the drop to air by removing 

 the >liding t ]> from the ga> chamber. Are the movements renewed ? 

 Try the same experiment with I'aramecia that have entirely ceased 

 movement. 



j<)i>. l-'ficcts nf curium tiio.viilc on I'araniccinni. ( ienerate the 

 gas with marble and dilute IK'l. Pass through two wash bottles 

 with water, and through the chamber as before. 



Study the effects of (. '( ).. as abnve. -Vote carefully any differences 

 from the IL experiment. Determine the reversibility of the effects 

 as before. 



297. liffcct.f f lack of o.vyt/cn and carl'on dio.vidc on the 

 ciliatcil epithelium of the oyster /////. Mount jiortions of gill fila- 

 ments in s L - a -water in the Kngelmann gas chamber as above, and 

 study the effects produced by a stream of hydrogen and of O ), 

 respectively. .Vote carefully all differences between the effects of 

 these two gas(.-~. ( "oinpare with the conditions in 1'aramecium. 





