Effect of salts on aggregation 



(51 



would resolve themselves into Hb 2 and Hb 2 4 as being the more stable 

 arrangement. 



And now does the theory fit the facts ? 



FIG. 31. Dissociation curves of haemoglobin solutions in NaoHPO 4 , NaHC0 3 , KC1 and 



Kx n 

 NaCl. Points actually determined. Curves drawn from formula z//100 = - j^ n - 



Ordinate = percentage saturation with oxygen. Abscissa = oxygen pressure in mm. 



First let me consider the simplest cases. Below is a table of the 

 values of K and n which correspond to the various solutions of 

 haemoglobin which have been investigated. 



