The call for oxygen considered as a physiological test 119 



1.10. Blood samples collected, Stage I. 



1.18. Injected 50 c.c. MySOi solution (4-697 %). 



1.25. Increase of fluid in intestine 0'5 c.c. per min. 



1.32. ,, ,, ,, 0-5 c.c. per min. No movements to be seen. 



1.39. B.F1. = 27"8 c.c. per min. = 0'397 c.c. per grm. per min. B.P. =110. 



1.40. Blood samples taken, Stage II. 



1.46. 15 c.c. of fluid removed from the intestine. 



1.51. Increase of intestine volume 0'25 c.c. per min. 

 2 09. ,, ,, ,, 0*01 c.c. per min. 



2.10. B.F1. =27-8 c.c. per min. =0'397 c.c. per grm. per min. B.P. = 105. 



2.11. Blood samples taken, Stage III. 

 2.15. Animal killed. 



64 c.c. of fluid were recovered from the intestine. The fluid was very viscid and had 

 much mucin in it. Alkaline in reaction and slightly blood stained. It contained 

 2-48 / MgS0 4 . The fluid removed at 1.46 was clear and contained 4-04 / MgS0 4 . 

 The intestine weighed 70 grms. The mucous membrane was very distinctly injected 

 throughout especially over all Peyer's patches. It was covered with thick mucus. The 

 solution injected contained 2-35 grm. MgS0 4 . That withdrawn 0-61 grm. and that 

 recovered at the end of the experiment 1-59 grm. This leaves 0-15 grm. unaccounted for. 

 This probably was partly absorbed, partly adherent to the mucous membrane although 

 the intestine was washed out and the sulphate estimated in the washings. The minimum 

 amount of water secreted into the intestine was 29 c.c. 



The samples on analysis gave the following results : 



Stage I Stage II Stage III 



Time 1.10* 1.40 2.11 



2 per grm. per min. ... 0-0191 0-0203 0-0166 c.c. 



Blood Flow ... 0-357 0-397 0-397 c.c. 



Time after injection ... 22' 53' 



* Injection at 1.18. 

 Or in percentages : 



2 100 106 87 



Blood Flow . 100 111 111 



REFERENCES 



(1) Barcroft and Straub, Journal of Physiol. xn, p. 145, 1910. 



(2) Starling, Ibid, xxiv, p. 317, 1899. 



(3) Knowlton. Ibid. XLIII, p. 219, 191*1. 



(4) Brodie and Vogt, Ibid. XL, p. 135, 1910. 



(5) Brodie, Cullis and Halliburton, Ibid. XL, p. 173, 1910. 



