INITIATION OF DEVELOPMENT IN CILETOPTERUS. 



TABLE VII. 



w/io Hydrochloric Acid. 



Ml 20 Sulphuric Acid. 



N/io Nitric Acid. 



a 5 drops + 100 c.c. sea- water i hr.-2 hr .-permanently 



b i c.c. + 99 c.c. sea-water I min.~5 min.-io>i min.-30 min.-i hr.-2 hr. 



permanently, 

 i min.-S min.-i5 min.-3i min.-i hr.-2 hr. 



permanently. 



c 5 c.c. + 95 c.c. sea-water 



Results: 



a gave no polar bodies or swimmers. 



b, 1-5 min. gave polar bodies no swimmers. 



b, 10^2 min. gave polar bodies and swimmers. 



c, 5 min. gave swimmers. 



n/2O Oxalic Acid. 



5 drops + 100 c.c. sea- water 

 i c.c. + 99 c.c. sea-water 



I hr. 2 hr.-permanently 

 i hr.-2 hr. permanently 



None 

 None 



None 

 None 



very little, if any, nuclear growth, but in each egg several asters 

 appeared, without spindles, and with a large, black-staining mass 

 of chromatin (?) at the center of the aster. There was more or 

 less breaking up to form "cells" either nucleate or a-nucleate, but 

 no real, mitotic cleavage. 



C. Alkali. The only alkali used was sodium hydroxide, and 

 as this was used in combination with sodium chloride it will be 

 described under the section on the combined use of two agents. 



D. Alcohol. Very little development followed the use of 



