A. J. GOLDFARB. 



TABLE 



No. Experiment. 



33 Centrifuged 35 X per min. for 



38 Centrifuged 35 X per min. for 



2"jc Centrifuged 35 X per min. for 

 20-2 Centrifuged 60 X per min. for 



25-10 Centrifuged 60 X per min. for 



II. Continued. 



Result. 



5 Developed normally. Some irreg. 



7^ Developed normally. Some irreg. 



10 Developed normally. More irreg. 



1 Developed normally. More irreg. 



2 Developed normally. 



2 Developed normally. 



3 Developed normally. 



5 Developed normally. More irreg. 



5 Yi Developed normally. More irreg. 



6 Developed normally. More irreg. 



7 Developed normally. More irreg. 

 6 Developed normally. More irreg. 

 6 Developed to young plutei only. 



Many irreg. 



8 Developed same. 

 10 Developed same. 



13 Developed irregularly. 



J^ Developed normally. 



1 Developed increasingly irreg. 



2 Developed increasingly irreg. 



4 Developed increasingly irreg. 



5 Developed increasingly irreg. 



2 Developed to gastrula. Mostdead. 



1 Developed normally. Some irreg. 



2 Developed normally. Some irreg. 

 4 Developed normally. Some irreg. 



6 Developed normally. Many irreg. 

 8 Developed normally. Most irreg. 



10 Developed normally. Most irreg. 



H Developed young plutei only. 



1 Developed increasingly irreg. 



2 Developed increasingly irreg. 



3 Developed increasingly irreg. 



4 Developed increasingly irreg. 



5 Developed increasingly irreg. 



In an effort to agglutinate the eggs, they were Centrifuged not 

 in urine tubes but in finely drawn glass capillaries, plugged at one 

 end with paraffin, and the eggs liberated a few minutes to 24 

 hours after centrifuging. This method was unsatisfactory, first, 

 because development ceased altogether within the capillaries, 

 and when liberated, after four or more hours, development was 

 atypical. When liberated, after 18 or more hours in the capil- 

 laries, they did not develop at all, Table III. Secondly, in 

 removing the eggs, it was necessary to exert considerable force, 



386 Centrifuged 60 X per min. for 

 18 Centrifuged 60 X per min. for 



25-20 Centrifuged 90 X per min. for 



