THE PRODUCTION OF GRAFTED EMBRYOS. 8 1 



THE EFFECT OF NAOH. 



The gelatinization of the egg by the addition of NaOH and 

 other alkalines to sea water aids materially in the agglutination. 

 \Yhen 25 drops or more of 0.5 per cent. NaOH solution was 

 added to 200 c.c. of sea water, the gelatinization was so great, 

 that after centrifuging, the eggs were distorted almost beyond 

 recognition. Figs. 6 and 7, on page 75, give an inadequate idea 

 of the degree of distortion. Less NaOH, namely, 4 to 10 drops in 

 the same amount of sea water, gave rise to no abnormalities. 



From the foregoing it is clear that w r ithin the limits specified, 

 the various steps found necessary in the production of aggluti- 

 nated eggs, either separately or all together, did not materially 

 effect development, except for the small fragments which gave 

 rise to atypic blastulac and gastrula?, already mentioned. Table 

 V. gives a list of experiments in which numerous agglutinated 

 and fused embryos were observed. 



TABLE V. 



This table gives a list of experiments in which agglutinated and fused eggs were 

 produced. 



Exp. No. Method Used. 



20.3 Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 5 min. 

 362 Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 

 3/>4 Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 12 

 25 C5 Fertilized, shaken, calcium-free solution, NaOH centrifuged 30 X, 6 

 c6 Fertilized, shaken, calcium-free solution, NaOH centrifuged 30 X, 8 



27 b Fertilized, shaken NaOH centrifuged 35 X, 6 



ce Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 5 



33 d Fertilized, shaken, calcium-free solution, NaOH centrifuged 30 X, 10 



34 d Fertilized, shaken, calcium-free solution, NaOH centrifuged 30 X. 



37 &3 Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 3 



Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 5 



Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 7 



di Fertilized, shaken, calcium-free solution, NaOH centrifuged 60 X, 2 



d2 Fertilized, shaken, calcium-free solution, NaOH centrifuged 35 X, 2 



d$ Fertilized, shaken, calcium-free solution, NaOH centrifuged 3 



</4 Fertilized, shaken, calcium-free solution, NaOH centrifuged 5 

 d$ Fertilized, shaken, calcium-free solution, NaOH centrifuged 



62 Fertilized, shaken, calcium-free solution, NaOH centrifuged 2 



38 3 Fertilized, shaken, calcium-free solution, NaOH centrifuged 2 

 dj Fertilized, shaken, calcium-free solution, NaOH centrifuged 5 

 d& Fertilized, shaken, calcium-free solution, NaOH centrifuged 7 



Etc. 



