140 EXPERIMENTAL PHARMACOLOGY 



the instructor) and fill the burette with brandy. What is 

 the percentage of alcohol in brandy? How is brandy made? 

 Start the drum and inject what you believe will be a fair 

 sized dose for vour animal. Do this two or three times 



/ 



and secure satisfactory tracings. How do these tracings 

 compare with those obtained from alcohol? 



3. Replace the brandy in the burette with whiskey. 

 What is the percentage of alcohol in whiskey? Inject a 

 fair sized dose and secure satisfactory tracings. This may 

 be done two or three times if the animal is sufficiently re- 

 sistant. But if the animal is weak, then the fewest injec- 

 tions possible should be given to secure typical tracings 

 showing the action of each drug. 



4. Substitute wine for the whiskey in the burette. How 

 much alcohol is there in wine? What else is contained in 

 wine? Inject one or two cubic centimeters and see how 

 this affects the animal. Use larger doses if you can safely 

 do so. How do the records obtained compare with those 

 from alcohol, brandy and whiskey? How do you explain 

 this? Inject some adrenaline and then allow the animal 

 to recover. 



5. Fill the burette with twenty per cent methyl alcohol. 

 Inject one cubic centimeter and secure a record. How 

 does the toxicity of this drug compare with that of ethyl 

 alcohol? If it appears safe, a larger dose may be injected 

 to secure satisfactory tracings. Does the alcohol which 

 you have injected affect the anesthesia in any Avay? 



6. Substitute a ten per cent mixture (shake well) oi ? 

 amyl alcohol and water for the methyl alcohol in the bu- 

 rette. Inject one-half cubic centimeter and secure trac- 

 ings. What can you say about the toxicity of this body .' 

 Kill the animal with amyl alcohol. Which stops first, the 

 heart or the respiration? Give some adrenaline, massage 

 the heart and give artificial respiration to see if you can 

 revive the animal. It is important for the student to learn 



