176 EXPERIMENTAL PHARMACOLOGY 



watch for any change in these. Allow the animal to walk 

 about the room and do not disturb it at first. How is the 

 respiration affected? Does the animal vomit! If so, can 

 yon detect morphine in the vomitns ? How is morphine ex- 

 creted? Is there present an increase in the secretion of 

 saliva? How do yon account for this? Does the animal 

 defecate? How is the intelligence of the dog affected? 

 What later changes do you note in the respiration? Are 

 there any symptoms of excitement? Take the rectal tem- 

 perature. When the animal lies down and becomes quiet, 

 then try its reflexes from time to time by pinching, loud 

 noises, etc. Eecord the respiration on the drum and watch 

 for irregularities (Cheyne-Stokes respiration). Are there 

 any changes in the pupils? Consult several text-books to 

 find out what action morphine has on the dog's pupil. 

 Would the administration of ether to a dog that had pre- 

 viously received a dose of morphine in any way affect the 

 action of the alkaloid on the pupil? Has the animal's tem- 

 perature changed? Keep the animal in a quiet place (in 

 a metabolism cage if possible) until next day and follow 

 the course of the drug's action as fully as you can. Is 

 the recovery complete? How long does this require? If 

 the animal dies what will be the immediate cause of death ? 

 If you succeed in collecting any urine test this with Bene- 

 dict's modification of Fehling's solution. Do you get a 

 positive test? What does this show? If you do not have 

 this solution use Fehling's or Haines' solution. 



EXPEKIMEXT XXVI. 

 Fehling's Test for Reducing Bodies. 



1. Into a test tube pour five cubic centimeters each of 

 solutions A and B. Bring the mixture to a boil. Is there 

 any change from a clear deep blue color? If so the solu- 

 tion is probably decomposed. If no change of color and 

 no precipitate forms the solution is satisfactory. Add 



