332 EXPERIMENTAL PHARMACOLOGY 



centimeter equals one milligram) of tetramethylammo- 

 niiim chloride. Inject one cubic centimeter and determine 

 what action this drug has after lobeline. What structures 

 are affected by the drug and how are they influenced ? Kill 

 the animal with a big dose of the tetramethylammonium 

 chloride. AVas the bladder contracted before the last drug- 

 was injected? If so, you may miss part of the action of 

 the drug. 



EXPERIMENT LXXXII. 



Lobeline, Nicotine, Pilocarpine. (Guinea Pig, Cat, Dog, or 



Rabbit: Uterus Strip.) 



(Consult also the following experiment. One animal may 

 be used for both experiments.) 



1. Examine carefully the apparatus shown in Fig. 292. 

 (Consult also Fig. 316). This simple arrangement is suf- 

 ficient for ordinary qualitative results but the more elab- 

 orate apparatus should be used for especially important 

 experiments such as drug assaying by the uterine strip 

 method. 



The animal used in this experiment may often be ob- 

 tained from another group of students who have already 

 performed another experiment. It is important to save as 

 many animals as possible. If a dog or cat is used it must 

 first be etherized, then one horn of the uterus (including 

 the ovary) is dissected out very carefully so as not to in- 

 jure the tissues. From this a small uterine strip is pre- 

 pared and with a bent pin hook one end of the uterine strip 

 is attached to the loAver end of the bent glass tube as shown 

 in the picture. By another pin hook and a small thread the 

 upper end of the tissue is attached to the short arm of the 

 heart lever. The lever is weighted on the long end by a 

 (movable) bull-dog clamp. If a guinea pig is used (and 

 these animals are very satisfactory) it may first be anes- 



