342 EXPERIMENTAL PHARMACOLOGY 



or muscarine or arecoline? What differences do YOU note? 



& 



Stimulate one vagus nerve to see if the heart can be in- 

 hibited. If so give another dose of hyoscine (or atropine 

 or hyoscyamine ) . 



If the animal is still in fair condition inject another dose 

 of physostigmine to see if the respiration, intestine and 

 circulation are affected in the same way as they were be- 

 fore the atropine was given. Do you observe any fine mus- 

 cular tremors over any parts of the animal? Were these 

 present before the atropine was given? Has the atropine 

 affected the tremors in any way! Explain. If you get a 

 marked intestinal reaction then inject one cubic centimeter 

 of adrenaline and see what occurs in the intestinal record. 

 Explain fully. 



Kill the animal with a large dose of eserine. Which stops 

 first, the heart or respiration? Save the animal for the 

 following experiment. 



EXPERIMENT XC. 



Adrenaline, Sodium Nitrite, Barium Chloride. (Dog 1 , Cat 

 or Rabbit: Perfusion of Kidney.) 



(See preceding experiment. Dog's kidneys are much to 



be preferred.) 



1. Observe carefully the arrangement of the apparatus 

 shown in Fig. 298. The water motor has a wire rod at- 

 tached eccentrically bv a screw to the end of the shaft. 



i- 



This rod passes up to the shelf above and ends in a curved 

 hook which moves rapidly up and down and thus inter- 

 rupts the flow of normal salt solution (or diluted, defibrin- 

 ated blood) from the pressure bottle (or bottles) above. 

 This mechanical interruption fairly closely approximates 

 the action of the heart in producing a pulsation in the 

 arteries carrying blood to the various organs. 



The apparatus should all be set up and in full working 



