346 EXPERIMENTAL PHARMACOLOGY 



The right kidney or the spleen of the same animal may 

 be used by other members of the class if sufficient appara- 

 tus is available. The motor and the pulsations in the per- 

 i'usion fluid may be omitted it' the apparatus cannot be ob- 

 tained. The interrupted pressure is. however, much to be 

 preferred as it is more effective in keeping up the circula- 

 tion through the organ (and edema of the tissues may be 

 prevented or much delayed in its appearance). The tin 

 box chamber (oncometer) may be omitted and the rate of 

 perfusion determined by counting the drops of solution 

 leaving the vein. It is advisable, however, to get a volume 

 record of the organ. 



Many forms of perfusion apparatus have been devised 

 and the student may find it necessary to use an outfit very 

 different from that here described. (For description of an 

 excellent perfusion apparatus, see Richards, A. X., and 

 Drinker, C. K.: Journal of Pharmacology and Experi- 

 mental Therapeutics, 1915, vii, pp. 467 to 483.) 



EXPERIMEXT XCI. 



Physostigmine, Atropine, (Heroine), Adrenaline. (Spinal 



Dog or Cat: Bronchioles, Blood-pressure, and 



Bladder Contractions.) 



1. Arrange a spinal dog (or cat if dogs are not avail- 

 able) for recording blood-pressure, bladder and bronchiole 

 contractions. It is very advisable that the lung records be 

 obtained by the aspiration method using the apparatus 

 shown in Fig. 255. Pith the animal by destroying the brain 

 through a trephine opening (Fig. 260). (Also read Experi- 

 ment LXX.) The arrangements for recording the bladder 

 contractions should be made (except attaching the record- 

 ing tambour as this would interfere with the operations) 

 before the apparatus used to hold the chest open is inserted 

 and this should be done (under positire artificial rexpira- 

 iion) before the animal is pithed, irliicli is the last opera- 



