358 



EXPERIMENTAL PHARMACOLOGY 



and mouth and the muzzle is connected to the apparatus 

 directly, the muzzle flange being slipped over the spout on 

 the pan. The connection is made air tight by a broad rub- 

 ber band. The pan is filled with pure X 2 and the anes- 

 thesia is brought on as rapidly as possible. Oxygen is 

 added in small quantities as needed. (The usual mistake 

 is in giving too much oxygen.) AVatch the pulse by keeping 



Fig. 304. View of the reverse side of the metallic muzzle shown in Fig. 303. The 

 flange which is not being used is closed by a rubber stopper. 



one finger over the left femoral artery all the time. The 

 heart will become slow but a little fresh oxygen will ac- 

 celerate it (this sometimes acts almost like giving adrena- 

 line so far as one can tell by feeling the pulse). 



As soon as the animal becomes quiet an assistant scrubs 

 an area about four inches in diameter over the lumbar por- 

 tion of the spinal column vigorously with soap and water. 

 The hair over the area is then clipped and the skin is shaved 

 closely. The area is washed thoroughly with alcohol and 

 allowed to dry. Tincture of iodine is then painted (with a 

 small cotton sponge) thoroughly over the area. 



An assistant now pours about half an ounce of the com- 

 pound cresol solution (lysol may be used) into the water in 

 the large pan. The operator mixes the cresol (or lysol) in 

 the water and thoroughly scrubs his hands in the solution. 



