KATE OF LYMPH FLOW I" IS.") 



any more than can be helped in putting in the bladder can- 

 nula. Clamp the penis or vulva with a hemostat to prevent 

 urination. 



Consult Figs. 319 and 302 and dissect out the thoracic 

 duct. Place a cannula in it and collect the lymph as it drops 

 from the small rubber tube placed on the cannula. The 

 cannula must be very small, the opening in the end should 

 be about the size of a very small thread and the outside of 

 the tip of the cannula should not exceed %2 or %4 of an inch 

 in diameter. Try to avoid penetrating the chest cavity at 

 the apex in dissecting out the duct. If the chest is opened it 

 may be necessary to give artificial respiration during the 

 rest of the experiment. If the cream has reached the in- 

 testine and is being absorbed well (two to three hours after 

 meal) the thoracic duct in the neck should be of a whitish or 

 yellowish-white color and in an average sized dog will be 

 about y 1(! or % 2 of an inch in diameter. The duct is com- 

 posed of exceedingly thin and delicate tissue and can be 

 easily torn off and, lost in the dissection, the student per- 

 haps not seeing it at all at any time. A little pressure will 

 compress the duct and check all out-flow from it. 



(Those students who have sufficient technical skill may 

 also insert a cannula in the lymphatic duct coming from the 

 head and collect lymph from both ducts at the same time. 

 There is a very marked difference and the experiment is 

 well worth doing.) 



The injecting burettes contain pituitrin (1 to 5 Parke, 

 Davis & Co.), ergamine (ten cubic centimeters equal one 

 milligram) and adrenaline (1:10,000). 



When all adjustments are made take a normal tracing, 

 watch the pupils and inject one cubic centimeter of pituitrin 

 solution (dog, eight kilos or more). Do you get a rise in 

 pressure, contraction of the bladder and a change 'in the 

 rate and depth of respiration? If the dose was sufficient, as 

 it probably was, alloAv the animal to return to normal and 

 wait until the normal rate of lymph flow is determined 



