PRELIMINARY OPERATIONS 



467 



two of plienolphtlialein solution. The injecting burettes 

 contain one-half per cent lactic acid and one per cent sodium 

 carbonate solution. 



Open the abdomen and with the greatest care not to 

 manipulate the intestines more than can be helped, pick 

 up the duodenum and with a hypodermic syringe inject 

 into the lumen of the gut five cubic centimeters of fluid- 

 extractum rhei, U.S.P. Replace this part of the intestine 

 and then going as far as possible down the small intestine 

 pick up the ileum and tie four ligatures around the gut as 

 shown in Fig. 357, thus isolating three loops of the intestine 

 each about three inches long. Into the first of these loops 



Fig. 357. Arrangement of the ligatures for isolating segments of the intestine in 



Moreau's experiment. 



(which should be tied with a colored ligature) inject five 

 cubic centimeters of normal salt solution. Into the middle 

 loop inject five cubic centimeters of normal salt solution to 

 which half a drop of croton oil has been added. Into the 

 third loop inject five cubic centimeters of twenty-five per 

 cent magnesium sulphate solution. Carefully replace the 

 intestine, close the abdomen and prepare to take some rec- 

 ords on the drum. If the animal is doing well it is prefer- 

 able to wait half an hour or so to allow the drugs in the 

 intestine to act. As soon as some urine is secreted observe 

 its color closely. Then add a little sodium hydrate and note 

 any changes. What are the active principles of rhubarb ? 



