172 EXPERIMENTAL PHARMACOLOGY 



EXPEKIMENT XX. 



Chloral Hydrate, Adrenaline. (Turtle's Heart.) 



1. Pith a turtle and take a normal heart tracing. Then 

 drop on the heart four per cent chloral hydrate solution 

 until the beats become slow. Then drop on adrenaline 

 solution (1:10,000) and note carefully any change in rate 

 or amplitude of the heart record. What structures are af- 

 fected by each drug? What conclusions can you draw 

 from the experiment? 



EXPERIMENT XXI. 



Chloral Hydrate and Alkalies. 



1. Into a test tube containing two cubic centimeters of 

 a four per cent chloral hydrate solution pour an equal 

 volume of potassium hydrate solution. What do you ob- 

 serve ? Smell the mixture. What do you note ? A reaction 

 represented by the following equation has taken place : 



CC1 3 CHO + KOH = CHC1 3 + HCOOK 



What bodies are formed? Are they soluble in water? 



EXPERIMENT XXII. 



Morphine. (Frog: Central Nervous System.) 



1. Into the anterior lymph sac of a frog inject one cubic 

 centimeter of four per cent morphine acetate solution. 

 (The sulphate or hydrochlorate of morphine may be used 

 instead.) Place the animal on moist cotton in a battery 

 jar and observe its actions. Try the reflexes from time 

 to time. Are the pupils affected? Is there any change in 

 the respiratory movements? How does the action of the 

 drug compare with that of nitrous oxide or chloral? If 

 the frog becomes completely narcotized do not cast it aside 



