DIURETINE AND AGUltlXr. 



EXPERIMENT L. 



Diuretine, (Sodium-theobromine-salicylate), Agurine, 

 ( Sodium-theobromine-acetate ) . ( Rabbit : Diuresis 

 and Respiration.) 



1. Give by stomach two grams of urethane dissolved in 

 twenty-five cubic centimeters of water to a good sized rab- 

 bit. Wait ten minutes for the drug to be absorbed and 

 then give the animal a little ether to complete the anes- 

 thesia. Arrange to record (or collect or both) the drops 

 of urine as they fall from a bladder cannula (or from two 

 ureteral cannulas). Place a cannula in the femoral (or 

 jugular) vein and connect up a burette. Fill this with 

 diuretine one per cent (or agurine, one per cent). Arrange 

 to record the respiration. Count the pulse rate per minute. 



When all preparations are made wait ten or twenty min- 

 utes to obtain the normal rate of urine flow. Then inject 

 one-half cubic centimeter of diuretine [(Knoll and Com- 

 pany, 45 John St., New York) or agurine] and record the 

 effect on the respiration. Count the pulse rate and see if 

 it is affected. Now give more of the drug from time to 

 time and try to bring on the effect gradually, watching 

 carefully not to kill the animal by an overdose. What do 

 you observe! How do you account for this? If your drop 

 recorder does not work well then let a student operate 

 the recording signal magnet by means of a simple hand 

 key placed in series with a dry cell and the signal magnet. 

 The student can make and break the current each time a 

 drop falls. If you are skillful enough you can make a de- 

 vice to record each drop by electrical contact. But do not 

 spend too much time at this. 



When you have obtained as marked results as possible 

 from the diuretine (or agurine or both) then if the rabbit 

 is still in suitable condition fill the burette with one of the 



following solutions: 



