NICOTINE, ADRENALINE, PILOCARPINE 307 



and tie. Lift the ends of the ligature up and draw the lung 

 upward into view. Clamp the ends of the ligature to the 

 chest wall. Could you put a cannula into the artery (as 

 shown in the illustrations) while the heart was beating and 

 disturbing your operations considerably? 



EXPERIMENT LXXVI. 



Nicotine, Adrenaline, Pilocarpine, Atropine. (Dog: Blood- 

 pressure, Intraocular Pressure, Respiration and Kidney, 

 Spleen or Intestinal Loop Volume.) 



1. Give a dog a small dose of ehloretone and then ether- 

 ize it. Arrange for blood-pressure, respiration and kidney, 

 spleen or intestinal loop volume records. 



Observe the arrangement of the apparatus shown in Fig. 

 271. A medium sized syringe point is thrust into the an- 

 terior chamber of the eye as shown in the upper right hand 

 corner of the picture. The cornea is composed of tough, 

 dense tissue and the syringe point Avill not leak around the 

 outside if the needle is not moved from side to side after it 

 is inserted (at an acute angle) through the cornea. The 

 needle is attached to a rubber tube which connects with 

 a water manometer (filled with normal salt solution). The 

 needle and the end of the rubber tube are held in a hole in a 

 cork which is clamped in a burette clamp which can be 

 brought close to the eye. The manometer is filled with salt 

 solution from the mercury bulb and the tubes (washout 

 also) and needle are filled with the solution, a little of the 

 solution being allowed to run out of the needle as it is in- 

 serted through the cornea. It is advisable to use an extra 

 stand to hold the needle (burette clamp) close up to the 

 eye. The amount of pressure in the tube is regulated by 

 raising or lowering the manometer. The recording tam- 

 bour should be small. No fluid should be in the tube lead- 

 ing from the manometer to the tambour. The injecting 



