PERFUSIOX APPARATUS 



343 



condition with the water bath heated to 38 or 39 degrees 

 Centigrade before the animal is killed. Also a dish of warm 

 salt solution, three small cannulas (for the kidney vessels 

 and ureter), threads and a rubber bulb (1 or 2 oz.) carry- 

 ing a small pointed cannula should be prepared. The bull) 

 is used to flush out the vessels of the kidney immediately 



Cannula in 



-(.annula. in Vein 



annula in Ureter 

 fV Openma for osnnulas 



Fig. 298. Arrangement of apparatus for perfusion of an excised organ. (For discus- 

 sion see text.) 



after excision. This prevents clots from forming in the 

 capillaries and smaller vessels of the organ. 



As soon as the animal dies the abdomen is opened and 

 the left kidney is carefully excised, leaving the artery, vein 

 and ureter as long as possible. The kidney is immediately 

 transferred to the dish of warm salt solution, the rubber 



