ESERIXE, ADRENALINE, ATROPIXK 347 



tion done before the records are started. This sequence 

 of operative procedures is followed in order to save the 

 vitality of the animal as much as possible a spinal animal 

 may not stand operations well. The injecting- burettes 

 contain eserine (one cubic centimeter equals one milligram), 

 adrenaline (1:10,000), and heroine (one cubic centimeter 

 equals five milligrams other opium alkaloids, e. g., co- 

 deine, etc., may be used instead of heroine). 



When all apparatus is arranged take half an inch or 

 more of normal record and then inject one cubic centi- 

 meter (dog) of eserine. Do you get satisfactory bladder 

 and lung records ? A moderate dose of physostigmine has 

 been found to render the endings of the vagi nerves in the 

 bronchoconstrictor muscles more irritable than normally. 

 Allow the animal to recover as much as possible from the 

 drug and then stimulate each, or both, vagus nerves and 

 determine exactly what influence they possess over the cali- 

 ber of the bronchioles. Use varying strengths of the Fara- 

 dizing current and also try varying lengths of duration of 

 stimulation. It may be further instructive to use a series 

 of single shocks of varying strengths and duration to bring 

 out any bronchial changes. What conclusions can you 

 draw? (See Dixon and Brodie: Journal of Physiology, 

 1903, xxix, p. 119; also Dixon and Ransom, ibid, 1912.) 



Get the animal into as good condition as possible (give 

 adrenaline if necessary) and then inject a second dose of 

 eserine. When the effects come on inject one-half cubic 

 centimeter of adrenaline to counteract the eserine. Do 

 your records show normal results? \Vas the dose of 

 eserine of the right size? (Von must learn to estimate the 

 dose to suit your animal often the instructor can help 

 you here.) 



When the animal recovers give another dose of eserine 

 or, if a bronchial constriction returns after the adrenaline 

 effects wear off, omit tlie eserine and then inject one cubic 

 centimeter of atropine solution (one cubic centimeter equals 



