360 EXPERIMENTAL PHARMACOLOGY 



floor in a quiet place and watch it carefully. Is there any 

 disturbance of motion in the hind legs! If so does this ex- 

 tend to the fore legs also! After a few minutes test the 

 hind limbs for sensation and see if the animal feels pain 011 

 being pricked gently with a pin or stimulated with an in- 

 duced current, etc. Are there respiratory or cardiac dis- 

 turbances I Was the dose too large or too small ! Is there 

 any disturbance of heat or cold sensations in the hind 

 limbs? 



Keep the animal under a close observation for several 

 days, watching particularly for signs of infection or men- 

 ingitis. How long does it take for the effects of the drug 

 to pass off? // signs of infection appear the animal must 

 at once be chloroformed. 



On what structures within the spinal canal does the drug 

 act to produce the symptoms observed! 



Could you demonstrate this action on a mixed (sensory 

 and motor) nerve in any of the peripheral nerves of the 

 body? If so how would you do the experiment I Would the 

 vagus he a suitable nerve? 



EXPERIMENT XCIX. 

 Ergot. (Rooster: Action on Comb.) 



1. A white Leghorn rooster should be selected for the 

 experiment. The rooster should not be fed for twenty-four 

 hours before the experiment is performed. 



Examine the color, temperature and general appearance 

 of the comb and wattles. Then with a hypodermic syringe 

 inject deep into the breast muscles five cubic centimeters 

 of a fi.rst-class preparation of fluid extract of ergot 

 ( Squibb 's is usually satisfactory). (The drug may also be 

 given into the crop with a soft rubber catheter.) 



Place the animal in a quiet place and observe it carefully 

 from time to time for at least one or two hours. Do von 



