TYRAMIKE, ERGAMINE, ERGOTOXINE 363 



EXPERIMENT CL 

 Tyramine. (Frog: Capillary Circulation.) 



1. Repeat the above experiment on a fresh frog using a 

 solution of tyramine (parahydroxyphenylethylamine Bur- 

 roughs TVellcome and Co., New York). The solution may 

 be made up from the hypodermic tablets (one cubic centi- 

 meter equals four milligrams). Inject one cubic centimeter. 

 Repeat the observations and state your conclusions. AVhat 

 are the active principles found in ergot? (See Dale, Barger, 

 Laidlaw, Dixon: Journal of Physiology, xxxiv, xxxix, xli, 

 xliii ; Biochemical Journal, ii ; Archiv fur experimentelle 

 Pathologic und Pharmakologie, Ixi.) 



EXPERIMENT CIL 

 Ergamine. (Turtle: Lung Tracing.) 



1. Prepare a turtle to record a lung tracing. Take a nor- 

 mal and inject one cubic centimeter of ergamine solution 

 (five cubic centimeters equal one milligram) into the heart. 

 Record the results and discuss your conclusions in full. 

 Give different sized doses if necessar to et ood results. 





EXPERIMENT GUI. 



Ergotoxine, Ergamine, Adrenaline, Barium. (Dog or Cat: 

 Blood-pressure, Respiration, Uterine Contractions 



Barbour's Method.) 



1. Arrange a dog (or cat) for taking blood-pressure and 

 respiration tracings. Open the abdomen and gently move 

 the intestines upward toward the diaphragm. Expose the 

 uterine horns and ovaries. "\Vitli great care dissect the 

 ovaries loose from the underlying tissues and then gently 

 raise the two horns upward and slowly dissect loose the 



