392 EXPERIMENTAL PHARMACOLOGY 



This dose was probably large enough (for some animals 

 too large the instructor may advise you about this) but 

 if you think advisable try another dose to see if you can 

 get more satisfactory results. 



When the animal recovers give one cubic centimeter of 

 atropine, then stimulate the vagi and see if the heart is 

 inhibited (how are the bronchi affected?). Give a little 

 adrenaline to revive the animal and inject another dose 

 (estimate the size) of pituitrin. How are the bronchioles 

 affected now after the atropine? Would you advise the 

 use of pituitrin in bronchial asthma ! How do your results 

 in this experiment compare with those obtained on the 

 turtle lung ? Kill the animal with a large dose of barium 

 chloride solution (one-half per cent). How does this affect 

 the bronchi ? 



EXPERIMENT CXV. 



Pituitrin, Adrenaline, Aconitine. (Dog: Urine Secretion, 



Intestinal Contractions, Blood-pressure, and 



Respiration.) 



1. Arrange a dog for recording the blood-pressure, res- 

 piration and intestinal contractions (by the fingercot-bu- 

 rette method). The injecting burettes contain pituitrin (1 

 to 5) and adrenaline (1:10,000). Carefully isolate both 

 ureters (Fig. 162) and place a ureteral cannula (Fig. 213) 

 in each. Arrange the cannulas to collect the urine Mow 

 in a beaker. Record on the drum the rate of drop Mow with 

 a signal magnet (worked by a simple key in circuit with a 

 dry cell). Close the abdomen with hemostats and wait ten 

 or twenty minutes to get the normal rate of urine Mow. 

 (The drum should have a very slow speed.) 



When the normal rate of urine Mow has been determined 

 (if no urine is excreted after twenty minutes go on with the 

 experiment and watch for the Mow to begin), take a nor- 

 mal record and then inject one cubic centimeter (for eight 



