394 EXPERIMENTAL PHARMACOLOGY 



EXPERIMENT CXVI. 



Pituitrin, Adrenaline, Vanadium. (Dog: Pulmonary 



Blood-pressure.) 



1. In the manner described in Experiment CIV, p. 369 

 (also in Experiment LXXVII, p. 310) arrange a dog for 

 recording pulmonary blood-pressure. The injecting bu- 

 rettes contain pituitrin (1 to 5) and adrenaline (1:10,000). 

 When all adjustments are made take a normal record and 

 then inject one cubic centimeter pituitrin solution. Do you 

 get a change in pulmonary pressure? Would you advise 

 the use of pituitary extract in a pulmonary hemorrhage 

 from a tuberculous lesion ? What structures are affected 

 by the drug in the lungs! Would the drug be advisable in 

 bronchial asthma? 



After a rec-ord showing the action of pituitrin on the pul- 

 monary pressure has been obtained get the animal into as 

 good condition as possible and fill one burette with a solu- 

 tion of sodium orthovanadate (two per cent when the drug 

 is dissolved in the water the solution is slightly alkaline). 

 Add a very small amount of hydrochloric acid to neutralize 

 the alkalinity. A bright, clear, orange-yellow solution will 

 be produced. Take a normal record and inject two cubic 

 centimeters of the vanadium solution. Was the dose large 

 enough? If not, possibly you can still get another record 

 with a larger dose. Kill the animal with the vanadium 

 solution. What conclusions can you draw from the experi- 

 ment? On what structures does the vanadium act? 



EXPERIMENT CXVII* 

 Dissection of the Eye. Its Anatomy and Pharmacology. 



Consult Figs. 324 and 325. It is advisable to read the 

 ction on 

 anatomy. 



section on the anatomy of the eye in some good text-book on 



*It is expected that this experiment may be performed on a day when no other experi- 

 mental material is available. 



