406 EXPERIMENTAL PHARMACOLOGY 



ond dose may be given later.) Explain your results in de- 

 tail. 



Let the animal recover, take a normal and then inject one 

 cubic centimeter of sodium nitrite solution. Do you get a 

 satisfactory record? (If not a second dose may be given 

 later.) Explain your results in detail. For what purpose 

 do you infer nitrites might be used clinicallv ? 



/ 



If the animal is now in poor condition kill it with a large 

 dose of nitrite. How much is required? But if the animal 

 is in fair or good condition (as it probably will be) then al- 

 low it to recover as fully as possible and proceed to per- 

 form the next experiment on it. 



EXPERIMENT CXXIV. 



Nitrites, Pilocarpine, Adrenaline. (Dog: Bronchial 



Action.) 



1. Arrange to record bronchial contractions by one of the 

 methods previously given. (If the animal from Experi- 

 ment CXXIII is used remove the spleen or intestinal onco- 

 meter and close the abdomen tightly.) The injecting bu- 

 rettes contain one per cent nitroglycerine, pilocarpine (one 

 cubic centimeter equals one milligram) and adrenaline (1: 

 10,000). 



Pith the animal, adjust the recording instruments and 

 take a short normal record. Inject one cubic centimeter of 

 pilocarpine. This should produce a bronchoconstriction 

 which will last for several minutes if no other drugs are 

 given. When the bronchoconstriction becomes marked 

 then inject two cubic centimeters of nitroglycerine solution. 

 What action has this on the bronchioles'? Would you ad- 

 vise nitrites for bronchial asthma? (iive one-half cubic 

 centimeter of adrenaline. 



If the animal is still in suitable condition a further in- 

 jection of pilocarpine (to produce an initial bronchocon- 



