ANTIPYEINE AND /3-TETRAHYDRONAPHTHYLAMINE 449 



EXPERIMENT CLI. 

 Antipyrine. (Frog: General Action.) 



1. Into the anterior lyinpli sac of a frog inject two cubic 

 centimeters of antipyrine solution (one cubic centimeter 

 equals ten milligrams). Put the frog in a quiet place and 

 observe its actions closely. Are the reflexes stimulated or 

 depressed! Is there developed somnolence or convulsions? 

 How is the central nervous system affected? 



EXPERIMENT CLII. 



Antipyrine, /3-tetrahydronaphthylamine Hydrochloride. 

 (Dog: Respiration, Blood-pressure, Leg Volume.) 



1. Arrange a dog for recording blood-pressure, respira- 

 tion and the volume changes of the left hind leg (plethys- 

 mograph). Take the animal's temperature at ten minute 

 intervals. Place antipyrine solution (one cubic centimeter 

 equals ten milligrams) and /3-tetrahydronaphthylamine hy- 

 drochloride (one cubic centimeter equals five milligrams) 

 in the two injecting burettes (in the right femoral and left 

 external jugular veins). 



Take a normal record and then inject one cubic centi- 

 meter of antipyrine. How is the heart affected? The res- 

 piration? Do you get a change in leg volume! If so what 

 does this signify? AYait for the animal to return to normal. 



Inject two cubic centimeters of /?-tetrahydronaphthyla- 

 niine solution (watch the pupils and eyelids closely as this 

 is done). Do you get a change in leg volume? (The rub- 

 ber cuff must fit tightly to the skin of the leg which must 

 be shaven closely you cannot make the cuff hold air if the 

 hair is left on the leg. Avoid vaseline for tit is purpose.) 

 How does the action of the /?-tetrahydronaphthylamine com- 

 pare with that of antipyrine on the blood-pressure, res- 

 piration, temperature controlling mechanism and leg vol- 



